PBIO 8100:
Plant Molecular and Cellular Biology I
|
Fall 2006 |
|
MWF 10:10-11:00 am Rm 1503 |
|
Thurs 9:30-10:45 am Rm 2507 |
| Instructors: | Kelly
Dawe 542-1658/Office - Plant Sciences 4611 Susan Wessler 542-1870/Office - Plant Sciences 4510 |
| Grading: | Exams
are 75% (25% each) of the course grade. Discussion groups are 25% of the course grade. |
Discussion groups: Primary literature will be assigned and discussed on designated days. ***Each student is expected to understand the methods, interpretation of data, experimental design and controls, and choice of experimental system. You will be asked to voice your views and summarize figures or sections of the papers.
Assigned Readings: The assigned research papers are linked below. Lecture material will be derived primarily from review articles. NOTE: lecture notes are here to give you a glimpse of what will be discussed. I wouldn't print them out just yet because I may change them, and because I'll hand them out during class anyway.
Syllabus for Dawe portion
| Week 1: INTRO TO MENDEL | |
| 8/16 |
Lecture: Intro to Mendel (lecture notes) |
| 8/17 |
Paper:Creighton and McClintock (1931). A correlation of cytological and genetical crossing over in Zea mays. Proc. Nat. Acad. Sci. 17, 492-497 |
| 8/18 | Lecture: Chromosomal mutations and Mapping (lecture notes) |
| Week 2: CHROMOSOMAL MUTATIONS | |
| 8/21 |
Lecture: Point mutants (lecture notes) |
| 8/23 |
Paper: Kaya et al (2000). hosoba toge toge, a syndrome caused by a large chromosomal deletion associated with a T-DNA insertion in Arabidopsis. Plant Cell Physiol. 41, 1055-1066 |
| 8/24 |
Lecture: TDNA (lecture notes) |
| 8/25 |
Paper: Osakabe et al (2005). Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells. Plant Mol. Biol. 57: 819-833 |
| Week 3: T-DNA and TRANPSOSON-INDUCED MUTATIONS & CELL AUTONOMY | |
| 8/28 | Lecture: Unstable mutations and transposon tagging. Here is an excellent review that summarizes the lecture. |
| 8/30 |
Paper: Amai et al (2004). Spermidine synthase genes are essential for survival of Arabidopsis. Plant Physiol. 135, 1565-73 [T-DNA paper] |
| 8/31 | Paper: Kolesnik et al (2004). Establishing an efficient Ac/Ds tagging system in rice: large-scale analysis of Ds flanking sequences. Plant J. 37: 301-314 |
| 9/1 | Lecture: Cytoplasmic mutations and cell autonomy (lecture) |
| Week 4: CELL AUTONOMY & HOTHEAD | |
| 9/4 | Labor Day no class |
| 9/6 | Paper: Shikanai et al (2001). The chloroplast clpP gene, encoding a proteolytic subunit of ATP-dependent protease, is indispensable for chloroplast development in tobacco. Plant Cell Physiol. 42, 264-73. |
| 9/7 |
Paper: Dawe gone. Jinghua Shi will lead discussion. Lolle et al (2005). Genome-wide non-mendelian inheritance of extra-genomic information in Arabidopsis. Nature. 434, 505-9. |
| 9/8 |
no class |
| Week 5: DOMINANT MUTATIONS & ACTIVATION TAGGING | |
| 9/11 | Lecture: Dominant mutations (lecture) |
| 9/13 |
Paper: Gosti et al (1999). ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 11, 1897-1909 |
| 9/14 |
Paper/Lecture: Tani et al (2004). Activation tagging in plants: a tool for gene discovery. Funct Integr Genomics 4, 258-266 |
| 9/15 |
Paper: Koch et al (2006). A role for a flavin-containing mono-oxygenase in resistance against microbial pathogens in Arabidopsis. Plant J. 629-639 |
| Week 6: EPISTASIS | |
| 9/18 |
review :EXAMPLE QUESTIONS |
| 9/20 |
Lecture: Epistasis (lecture) |
| 9/21 |
Exam I |
| 9/22 | Paper: Luo and Oppenheimer (1999) Genetic control of trichome branch number in Arabidopsis: the roles of the FURCA loci. Development 126: 5547-5557 |
| Week 7: SUPPRESSORS and REVERSE GENETICS | |
| 9/25 |
Lecture: Suppressors (lecture) |
| 9/27 |
Paper: Steber et al. (1998) Isolation of the GA-response mutant sly1 as a suppressor of ABI1-1 in Arabidopsis thaliana. Genetics 149: 509-521 |
| 9/28 |
Lecture: Reverse Genetics (lecture), also review by Bouche and Bouchez (2001) |
| 9/29 |
Lecture: RNAi & microRNAs (lecture) see also this comprehensive poster on RNAi |
| Week 8: RNAi and Histone Code | |
| 10/2 | Paper: Deleris et al (2006). Hierarchical Action and Inhibition of Plant Dicer-Like Proteins in Antiviral Defense. Science 313: 68 -71 |
| 10/4 |
Lecture: sick |
| 10/5 |
Lecture: Paper: Deleris et al (2006) continued, Histone code and Centromeres (.ppt lecture) |
| 10/6 | Paper: Bastow et al (2004). Vernalization requires epigenetic silencing of FLC by histone methylation. Nature 427, 164-7. |
| Week 9: HISTONES, continued | |
| 10/9 | Now to: Dr. Wessler's half of the course for Monday: Green paper For Wednesday: Barbazuk paper |